U19 project three

project three:

in vivo EPR for after-the-fact measurement of dose

The overall objective is to meet a critical need: determining immediately after an incident whether individuals have received a radiation dose that could result in acute clinical symptoms. If the exposure conditions indicate that acute clinical effects are possible, there is an urgent need to be able to place individuals into appropriate dose categories rapidly and accurately, in order to guide therapeutic approaches and counseling.

The development of the technique has yielded a standard error of the mean as low as 50 cGy for repeated measurements of the same sets of teeth in vivo in a single volunteer.
Measurements in patients whose teeth have been irradiated in the course of their radiation therapy for their tumors have been started, with measurements made in four patients. Measurements also have been made in two subjects who were concerned that they may have received significant doses of radiation from other circumstances. The results of the measurements have been consistent with the estimated exposure doses.
The instrument for measurements at Rochester is expected to be delivered and made operational before the end of the current grant year (year 2)
The “helmet type” magnet from the subcontract for Trinity College is expected to arrive for testing before the end of the current grant year
The fingernail project continues to show great promise and is proceeding faster than in the original plan.

Project 3 is developing biodosimeters to quantitatively assess the physical dose received as a result of radiation exposure. Potentially, these biodosimeters will make use of teeth, hair, and nails, and therefore should have broad utility in an accident scenario, where a variety of diagnostic tests and patient assessment tools will be needed. The method is now well established and primary efforts are now focused on implementation of in vivo instruments. The procedures for EPR dosimetry of teeth in the mouths of volunteers have been refined so that the data collection time currently is less than five minutes. Separate funding from the DOD was obtained that will accelerate our ability to create both fixed and mobile units. This will make it possible to supply, ahead of schedule, a fixed and improved instrument to Rochester to accelerate the clinical testing. Clinical protocols have already been written for testing in Rochester next year, and they are under review by the Internal Review Board. This improved instrument has obvious implications for interaction with Projects 4 and 5, serving to validate results of the assays for radiation exposure that are under development.

Project Three Personnel

Project 3: Communications & Publications

• R.A. Kleinerman, A.A. Romanyuka, D.A. Schauer, and J.D. Tucker, “Retrospective Assessment of Radiation Exposure using Biological Dosimetry: Chromosome Painting, Electron Paramagnetic Resonance and the Glycophorin - A Mutation Assay.” Radiat. Res. 166, 287-302 (2006).
• A.A. Romanyukha, D.A. Schauer and Y.K. Malikov, “Analysis of Current Assessments and Perspectives of ESR Tooth Dosimetry for Radiation Dose Reconstruction of the Population Residing Near the Semipalatinsk Nuclear Test Site.” J. Radiat. Res. 47, A55-A60 (2006).
• E. Tielewuhan, K. Tanaka, S. Toyoda, A. Kadoma, S. Endo, A. Romanyukha, O. Tarasov and M. Hoshi, “90Sr Concentration in Cow Teeth from South Ural Region, Russia, Using Monte Carlo Simulation.” J. Radiat. Res. 47, A117-A120 (2006).
• S. Toyoda, H. Imata, A. Romanyukha and M. Hoshi, “Toward High Sensitivity ESR Dosimetry of Mammal Teeth: The Effect of Chemical Treatment.” J. Radiat. Res., 47, A71-A74 (2006).
• A. Wieser, E. Vasilenko, P. Fattibene, S. Bayankin, N. El-Faramawy, D. Ivanov, P. Jacob, V. Knyazev, S. Onori, M.C. Pressello, A. Romanyukha, M. Smetanin and A. Ulanovsky, “Comparison of EPR Occupational Lifetime External Dose Assessments for Mayak Nuclear Workers and Film Badge Dose Data.” Radiat Environ Biophys. 44, 279-288 (2006)

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	project three: in vivo EPR for after-the-fact measurement of dose

		The overall objective is to meet a critical need: determining immediately after an incident whether individuals have received a radiation dose that could result in acute clinical symptoms. If the exposure conditions indicate that acute clinical effects are possible, there is an urgent need to be able to place individuals into appropriate dose categories rapidly and accurately, in order to guide therapeutic approaches and counseling.








			The development of the technique has yielded a standard error of the mean as low as 50 cGy for repeated measurements of the same sets of teeth in vivo in a single volunteer. Measurements in patients whose teeth have been irradiated in the course of their radiation therapy for their tumors have been started, with measurements made in four patients. Measurements also have been made in two subjects who were concerned that they may have received significant doses of radiation from other circumstances. The results of the measurements have been consistent with the estimated exposure doses. The instrument for measurements at Rochester is expected to be delivered and made operational before the end of the current grant year (year 2) The “helmet type” magnet from the subcontract for Trinity College is expected to arrive for testing before the end of the current grant year The fingernail project continues to show great promise and is proceeding faster than in the original plan.






			Project 3 is developing biodosimeters to quantitatively assess the physical dose received as a result of radiation exposure. Potentially, these biodosimeters will make use of teeth, hair, and nails, and therefore should have broad utility in an accident scenario, where a variety of diagnostic tests and patient assessment tools will be needed. The method is now well established and primary efforts are now focused on implementation of in vivo instruments. The procedures for EPR dosimetry of teeth in the mouths of volunteers have been refined so that the data collection time currently is less than five minutes. Separate funding from the DOD was obtained that will accelerate our ability to create both fixed and mobile units. This will make it possible to supply, ahead of schedule, a fixed and improved instrument to Rochester to accelerate the clinical testing. Clinical protocols have already been written for testing in Rochester next year, and they are under review by the Internal Review Board. This improved instrument has obvious implications for interaction with Projects 4 and 5, serving to validate results of the assays for radiation exposure that are under development.

Project Three Personnel








Project 3: Communications & Publications • R.A. Kleinerman, A.A. Romanyuka, D.A. Schauer, and J.D. Tucker, “Retrospective Assessment of Radiation Exposure using Biological Dosimetry: Chromosome Painting, Electron Paramagnetic Resonance and the Glycophorin - A Mutation Assay.” Radiat. Res. 166, 287-302 (2006). • A.A. Romanyukha, D.A. Schauer and Y.K. Malikov, “Analysis of Current Assessments and Perspectives of ESR Tooth Dosimetry for Radiation Dose Reconstruction of the Population Residing Near the Semipalatinsk Nuclear Test Site.” J. Radiat. Res. 47, A55-A60 (2006). • E. Tielewuhan, K. Tanaka, S. Toyoda, A. Kadoma, S. Endo, A. Romanyukha, O. Tarasov and M. Hoshi, “90Sr Concentration in Cow Teeth from South Ural Region, Russia, Using Monte Carlo Simulation.” J. Radiat. Res. 47, A117-A120 (2006). • S. Toyoda, H. Imata, A. Romanyukha and M. Hoshi, “Toward High Sensitivity ESR Dosimetry of Mammal Teeth: The Effect of Chemical Treatment.” J. Radiat. Res., 47, A71-A74 (2006). • A. Wieser, E. Vasilenko, P. Fattibene, S. Bayankin, N. El-Faramawy, D. Ivanov, P. Jacob, V. Knyazev, S. Onori, M.C. Pressello, A. Romanyukha, M. Smetanin and A. Ulanovsky, “Comparison of EPR Occupational Lifetime External Dose Assessments for Mayak Nuclear Workers and Film Badge Dose Data.” Radiat Environ Biophys. 44, 279-288 (2006)


about these images		contact us		website amy k. huser